Recombinant
RabMAb

Anti-DNAH3 antibody [EPR11746] (ab172632)

Overview

  • Product name
    Anti-DNAH3 antibody [EPR11746]
  • Description
    Rabbit monoclonal [EPR11746] to DNAH3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNAH3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8TD57

  • Positive control
    • Mouse testis lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11746
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 471 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
    • Tissue specificity
      Expressed primarily in trachea and testis, 2 tissues containing axonemal structures. Also expressed in lung.
    • Sequence similarities
      Belongs to the dynein heavy chain family.
    • Domain
      Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
    • Cellular localization
      Cytoplasm > cytoskeleton > cilium axoneme.
    • Information by UniProt
    • Database links
    • Alternative names
      • axonemal antibody
      • Axonemal beta dynein heavy chain 3 antibody
      • Ciliary dynein heavy chain 3 antibody
      • Dnah3 antibody
      • Dnahc3-b antibody
      • DYH3_HUMAN antibody
      • Dynein heavy chain 3 antibody
      • HsADHC3 antibody
      see all

    Images

    • Anti-DNAH3 antibody [EPR11746] (ab172632) at 1/1000 dilution + Mouse testis lysate at 10 µg

      Predicted band size: 471 kDa

    References

    ab172632 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172632.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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