Overview

  • Product nameAnti-DNAH5 antibody
    See all DNAH5 primary antibodies
  • Description
    Rabbit polyclonal to DNAH5
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Rhesus monkey, Gorilla
  • Immunogen

    Recombinant fragment : NFDTLTSSIN ARANALLLTT VTRKKKETEM LGEEARELLS HFNHQNMDAL LKVTRNTLEA IRKRIHSSHT INFRDSNSAS NMKQNSLP, corresponding to internal sequence amino acids 921-1008 of Human DNAH5 (Q8TE73)

  • Positive control
    • Human fallopian tube tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionForce generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles.
  • Involvement in diseaseDefects in DNAH5 are the cause of primary ciliary dyskinesia type 3 (CILD3) [MIM:608644]. CILD3 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
    Defects in DNAH5 are a cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
  • Sequence similaritiesBelongs to the dynein heavy chain family.
  • DomainDynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • Cellular localizationCytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Axonemal beta dynein heavy chain 5 antibody
    • Ciliary dynein heavy chain 5 antibody
    • DNAH 5 antibody
    • Dnah5 antibody
    • DNAHC5 antibody
    • DYH5_HUMAN antibody
    • Dynein heavy chain 5 antibody
    • Dynein heavy chain 5, axonemal antibody
    • HL1 antibody
    • KIAA1603 antibody
    see all

Anti-DNAH5 antibody images

  • ab122390 at 1/10 staining DNAH5 in formalin fixed paraffin embedded Human fallopian tube using immunohistochemistry.

References for Anti-DNAH5 antibody (ab122390)

ab122390 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"