Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAH7
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LKLRCERFVE ELESYAKQSE EFYSFGDLQD VQRYLKKAQI LNGKLDLAAD KIEQFNAEEE AFGWLPSVYP QRKKIQDGLN PYLRLYETAV EFSSNY, corresponding to amino acids 685-780 of Human DNAH7 (UniProt ID: Q8WXX0).

  • Positive control
    • Human fallopian tube tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
  • Tissue specificity
    Detected in brain, testis and trachea. Detected in bronchial cells (at protein level).
  • Sequence similarities
    Belongs to the dynein heavy chain family.
  • Domain
    Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • Cellular localization
    Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Axonemal beta dynein heavy chain 7 antibody
    • Ciliary dynein heavy chain 7 antibody
    • DNAH7 antibody
    • DYH7_HUMAN antibody
    • Dynein axonemal heavy chain 7 antibody
    • Dynein heavy chain 7 antibody
    • Dynein heavy chain-like protein 2 antibody
    • HDHC2 antibody
    • KIAA0944 antibody
    see all

Images

  • ab122226, at a 1/75 dilution, staining DNAH7 in paraffin embedded Human fallopian tube tissue by Immunohistochemistry.

References

ab122226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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