Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAJB11
  • Specificity
    ab75107 detects endogenous levels of total DNAJB11 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from an internal region of human DNAJB11.

  • Positive control
    • IHC-P: human heart tissue. WB: extracts from HeLa cells, HUVEC cells, COLO cells and MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 J domain.
  • Post-translational
    modifications
    Contains high-mannose Endo H-sensitive carbohydrates.
    Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
    Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
  • Cellular localization
    Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABBP-2 antibody
    • ABBP2 antibody
    • APOBEC1 binding protein 2 antibody
    • APOBEC1-binding protein 2 antibody
    • DJ9 antibody
    • DJB11_HUMAN antibody
    • DnaJ (Hsp40) homolog subfamily B member 11 antibody
    • DnaJ homolog subfamily B member 11 antibody
    • DnaJ protein homolog 9 antibody
    • DNAJB11 antibody
    • EDJ antibody
    • ER associated DNAJ antibody
    • ER associated dnaJ protein 3 antibody
    • ER associated Hsp40 co chaperone antibody
    • ER-associated DNAJ antibody
    • ER-associated dnaJ protein 3 antibody
    • ER-associated Hsp40 co-chaperone antibody
    • ERdj3 antibody
    • ERJ3 antibody
    • ERj3p antibody
    • hDj-9 antibody
    • hDj9 antibody
    • HEDJ antibody
    • Human DnaJ protein 9 antibody
    • PWP1 interacting protein 4 antibody
    • PWP1-interacting protein 4 antibody
    see all

Anti-DNAJB11 antibody images

  • All lanes : Anti-DNAJB11 antibody (ab75107) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HUVEC cells
    Lane 3 : extracts from COLO cells
    Lane 4 : extracts from MCF-7 cells
    Lane 5 : extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Immunohistochemical analysis of DNAJB11 in paraffin embedded human heart tissue using 1/50 ab75107.
    Left image: untreated sample.
    Right image: sample treated with immunising peptide.

References for Anti-DNAJB11 antibody (ab75107)

This product has been referenced in:
  • Croner RS  et al. Quantitative proteome profiling of lymph node-positive vs. -negative colorectal carcinomas pinpoints MX1 as a marker for lymph node metastasis. Int J Cancer 135:2878-86 (2014). Read more (PubMed: 24771638) »
  • Xu W  et al. Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis. Biol Reprod 89:142 (2013). Read more (PubMed: 24198121) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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