Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAJB12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 82-353 of Human DNAJB12 (Uniprot ID Q9NXW2)

  • Positive control
    • A549, H1299, HCT116 and MCF7 cell lysates; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling DNAJB12 using ab154410 at a 1/500 dilution.
  • All lanes : Anti-DNAJB12 antibody (ab154410) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10% SDS PAGE

References

ab154410 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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