Overview

  • Product nameAnti-DNAJB2 antibody
    See all DNAJB2 primary antibodies
  • Description
    Mouse monoclonal to DNAJB2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: LELSRREQQP SVTSRSGGTQ VQQTPASCPL DSDLSEDEDL QLAMAYSLSE MEAAGKKPAG GREAQHRRQG RPKAQHQDPG LGGTQEGARG EATKRSPSPE EKASRCLIL, corresponding to amino acids 216-324 of Human DNAJB2 (NP_006727) with a tag.

  • Positive control
    • A431 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 0.3ng/ml as a capture antibody when used against the immunogen.

Target

  • RelevanceDNAJB2 is involved in heat shock protein and unfolded protein binding.
  • Database links
  • Alternative names
    • Descriptions antibody
    • DnaJ (Hsp40) homolog subfamily B member 2 antibody
    • DnaJ homolog subfamily B member 2 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJB2 antibody
    • Heat shock 40 kDa protein 3 antibody
    • Heat shock protein J1 antibody
    • Heat shock protein neuronal DNAJ like 1 antibody
    • HSJ 1 antibody
    • HSJ1 antibody
    • HSPF3 antibody
    see all

Anti-DNAJB2 antibody images

  • Anti-DNAJB2 antibody (ab77322) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-DNAJB2 antibody (ab77322) at 5 µg/ml + A431 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab77322 was used as a capture antibody and detection limit for recombinant tagged DNAJB2 is approximately 0.3ng/ml.

References for Anti-DNAJB2 antibody (ab77322)

ab77322 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"