Overview

  • Product nameAnti-DNAJB6 antibody
    See all DNAJB6 primary antibodies
  • Description
    Rabbit polyclonal to DNAJB6
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Drosophila melanogaster
  • Immunogen

    A synthetic peptide corresponding to a region of Human DNAJB6

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36, 50 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

Target

  • FunctionPlays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
  • Tissue specificityWidely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
  • Involvement in diseaseLimb-girdle muscular dystrophy 1E
  • Sequence similaritiesContains 1 J domain.
  • DomainThe antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
  • Cellular localizationCytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJ4 antibody
    • DnaJ antibody
    • DnaJ (Hsp40) homolog subfamily B member 6 antibody
    • DNAJ B6 antibody
    • DnaJ homolog subfamily B member 6 antibody
    • DnaJ like 2 protein antibody
    • DNAJB6 antibody
    • DNJB6_HUMAN antibody
    • Heat shock protein J2 antibody
    • HHDJ1 antibody
    • HSJ-2 antibody
    • HSJ2 antibody
    • LGMD1E antibody
    • MRJ antibody
    • MSJ-1 antibody
    • MSJ1 antibody
    see all

Anti-DNAJB6 antibody images



  • Predicted band size : 36 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling DNAJB6 with ab75196 at 1.0µg/ml.
  • Anti-DNAJB6 antibody (ab75196) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Predicted band size : 36 kDa
    Observed band size : 36,50 kDa (why is the actual band size different from the predicted?)

References for Anti-DNAJB6 antibody (ab75196)

ab75196 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"