Overview

  • Product nameAnti-DNAJB6 antibody
    See all DNAJB6 primary antibodies
  • Description
    Rabbit polyclonal to DNAJB6
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 126 of Human DNAJB6 (NP_490647).

  • Positive control
    • HeLaS3 and MOLT4 cell lysates for WB and HeLa cells for IF

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionPlays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
  • Tissue specificityWidely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
  • Involvement in diseaseLimb-girdle muscular dystrophy 1E
  • Sequence similaritiesContains 1 J domain.
  • DomainThe antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
  • Cellular localizationCytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJ4 antibody
    • DnaJ antibody
    • DnaJ (Hsp40) homolog subfamily B member 6 antibody
    • DNAJ B6 antibody
    • DnaJ homolog subfamily B member 6 antibody
    • DnaJ like 2 protein antibody
    • DNAJB6 antibody
    • DNJB6_HUMAN antibody
    • Heat shock protein J2 antibody
    • HHDJ1 antibody
    • HSJ-2 antibody
    • HSJ2 antibody
    • LGMD1E antibody
    • MRJ antibody
    • MSJ-1 antibody
    • MSJ1 antibody
    see all

Anti-DNAJB6 antibody images

  • All lanes : Anti-DNAJB6 antibody (ab96539) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
  • ab96539, at 1/200, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).

References for Anti-DNAJB6 antibody (ab96539)

ab96539 has not yet been referenced specifically in any publications.

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