Overview

  • Product name
  • Description
    Goat polyclonal to DNAJB9
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    RGNMVTTYTDCSGQ

    , corresponding to C terminal amino acids 210-223 of Human MDG1.

  • Positive control
    • Human kidney
  • General notes
    GenBank Accession Number – NP_036460.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000. This antibody gave a positive result in ELISA against the immunizing peptide.
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa. Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1µg/ml.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as a co-chaperone with an Hsp70 protein.
  • Sequence similarities
    Contains 1 J domain.
  • Cellular localization
    Cytoplasm. Nucleus. Stress induces its translocation to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP564F1862 antibody
    • DnaJ (Hsp40) homolog subfamily B member 9 antibody
    • DnaJ homolog subfamily B member 9 antibody
    • DNAJB9 antibody
    • DNJB9_HUMAN antibody
    • eEndoplasmic reticulum DNA J domain containing protein 4 antibody
    • Endoplasmic reticulum DnaJ homolog 4 antibody
    • ER resident protein ERdj4 antibody
    • ERdj4 antibody
    • MDG 1 antibody
    • Mdg-1 antibody
    • MDG1 antibody
    • Microvascular endothelial differentiation gene 1 antibody
    • Microvascular endothelial differentiation gene 1 protein antibody
    • MST049 antibody
    • MSTP049 antibody
    • OTTHUMP00000206840 antibody
    see all

Images

  • ab6053 at 4 µg/ml staining MDG1 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Detected with HRP-staining.

References

ab6053 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us for technical support. I'm sorry to hear that ab6053 is not working well for you. This antibody is a FastTrack product and as such has not been fully characterised yet by our laboratory as we do not have good positive con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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