Overview

  • Product name
  • Description
    Goat polyclonal to DNAJB9
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    RGNMVTTYTDCSGQ

    , corresponding to C terminal amino acids 210-223 of Human MDG1.

  • Positive control
    • Human kidney
  • General notes
    GenBank Accession Number – NP_036460.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000. This antibody gave a positive result in ELISA against the immunizing peptide.
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa. Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1µg/ml.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as a co-chaperone with an Hsp70 protein.
  • Sequence similarities
    Contains 1 J domain.
  • Cellular localization
    Cytoplasm. Nucleus. Stress induces its translocation to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP564F1862 antibody
    • DnaJ (Hsp40) homolog subfamily B member 9 antibody
    • DnaJ homolog subfamily B member 9 antibody
    • DNAJB9 antibody
    • DNJB9_HUMAN antibody
    • eEndoplasmic reticulum DNA J domain containing protein 4 antibody
    • Endoplasmic reticulum DnaJ homolog 4 antibody
    • ER resident protein ERdj4 antibody
    • ERdj4 antibody
    • MDG 1 antibody
    • Mdg-1 antibody
    • MDG1 antibody
    • Microvascular endothelial differentiation gene 1 antibody
    • Microvascular endothelial differentiation gene 1 protein antibody
    • MST049 antibody
    • MSTP049 antibody
    • OTTHUMP00000206840 antibody
    see all

Anti-DNAJB9 antibody images

  • ab6053 at 4 µg/ml staining MDG1 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Detected with HRP-staining.

References for Anti-DNAJB9 antibody (ab6053)

ab6053 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us for technical support. I'm sorry to hear that ab6053 is not working well for you. This antibody is a FastTrack product and as such has not been fully characterised yet by our laboratory as we do not have good positive con...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up