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RGNMVTTYTDCSGQ, corresponding to C terminal amino acids 210-223 of Human MDG1.
Our Abpromise guarantee covers the use of ab6053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/4000. This antibody gave a positive result in ELISA against the immunizing peptide.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 26 kDa. Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1µg/ml.|
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab6053 has not yet been referenced specifically in any publications.