The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceDNAJC10 (DnaJ homolog, subfamily C, member 10) is a co-chaperone which is thought to have a role in protein folding and translocation across the endoplasmic reticulum membrane. It may act as a co-chaperone for HSPA5.