Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAJC14
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 396-542 of Human DNAJC14.

  • Positive control
    • Human stomach tissue; RT4, U 251 MG, Human Liver and Tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Regulates the export of target proteins, such as DRD1, from the endoplasmic reticulum to the cell surface.
  • Tissue specificity
    Highly expressed in pancreas and selectively expressed in brain, lung, liver, skeletal muscle and kidney.
  • Sequence similarities
    Contains 1 J domain.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJC14_HUMAN antibody
    • DnaJ (Hsp40) homolog, subfamily C, member 14 antibody
    • DNAJ antibody
    • DnaJ homolog subfamily C member 14 antibody
    • DnaJ protein homolog 3 antibody
    • DNAJC14 antibody
    • Dopamine receptor interacting protein antibody
    • Dopamine receptor-interacting protein of 78 kDa antibody
    • DRiP78 antibody
    • hDj 3 antibody
    • HDJ-3 antibody
    • HDJ3 antibody
    • Human DnaJ protein 3 antibody
    • LIP6 antibody
    • LYST interacting protein LIP6 antibody
    see all

Images

  • ab121535 at 1/375 dilution staining DNAJC14 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli using ab121535.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100
  • All lanes : Anti-DNAJC14 antibody (ab121535) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

References

ab121535 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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