Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAJC21
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

Applications

Our Abpromise guarantee covers the use of ab86434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May act as a co-chaperone for HSP70.
    • Tissue specificity
      Brain, placenta, kidney and pancreas.
    • Sequence similarities
      Contains 2 C2H2-type zinc fingers.
      Contains 1 J domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • DJC21_HUMAN antibody
      • DnaJ homolog subfamily A member 5 antibody
      • DnaJ homolog subfamily C member 21 antibody
      • DNAJA5 antibody
      • dnajc21 antibody
      • GS3 antibody
      • JJJ1 antibody
      • Protein GS3 antibody
      see all

    Anti-DNAJC21 antibody images

    • All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 62 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.

    References for Anti-DNAJC21 antibody (ab86434)

    This product has been referenced in:
    • Tummala H  et al. DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation. Am J Hum Genet 99:115-24 (2016). Read more (PubMed: 27346687) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab86434.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up