Overview

  • Product nameAnti-DNAJC21 antibody
    See all DNAJC21 primary antibodies
  • Description
    Rabbit polyclonal to DNAJC21
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

Applications

Our Abpromise guarantee covers the use of ab86434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay act as a co-chaperone for HSP70.
    • Tissue specificityBrain, placenta, kidney and pancreas.
    • Sequence similaritiesContains 2 C2H2-type zinc fingers.
      Contains 1 J domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • DJC21_HUMAN antibody
      • DnaJ homolog subfamily A member 5 antibody
      • DnaJ homolog subfamily C member 21 antibody
      • DNAJA5 antibody
      • dnajc21 antibody
      • GS3 antibody
      • JJJ1 antibody
      • Protein GS3 antibody
      see all

    Anti-DNAJC21 antibody images

    • All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 62 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.

    References for Anti-DNAJC21 antibody (ab86434)

    ab86434 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"