Overview

  • Product nameAnti-DNAJC3 antibody [EP597Y]
    See all DNAJC3 primary antibodies
  • Description
    Rabbit monoclonal [EP597Y] to DNAJC3
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human DNAJC3.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab52899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionInvolved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
    • Tissue specificityWidely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
    • Sequence similaritiesContains 1 J domain.
      Contains 9 TPR repeats.
    • DomainThe J domain mediates interaction with HSPA8.
    • Cellular localizationEndoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • DnaJ (Hsp40) homolog subfamily C member 3 antibody
      • DnaJ homolog subfamily C member 3 antibody
      • DNAJC3 antibody
      • DNJC3_HUMAN antibody
      • double-stranded RNA-activated protein kinase inhibitor antibody
      • Endoplasmic reticulum DnaJ protein 6 antibody
      • ERdj6 antibody
      • HP58 antibody
      • Interferon induced, double stranded RNA activated protein kinase inhibitor antibody
      • Interferon-induced antibody
      • P58 antibody
      • p58 ipk antibody
      • P58IPK antibody
      • PRKRI antibody
      • Protein kinase inhibitor of 58 kDa antibody
      • Protein kinase inhibitor p58 antibody
      • Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody
      see all

    Anti-DNAJC3 antibody [EP597Y] images

    • Anti-DNAJC3 antibody [EP597Y] (ab52899) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)

    References for Anti-DNAJC3 antibody [EP597Y] (ab52899)

    ab52899 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab52899.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"