Recombinant
RabMAb

Anti-DNAJC3 antibody [EP597Y] (ab52899)

Overview

  • Product name
    Anti-DNAJC3 antibody [EP597Y]
    See all DNAJC3 primary antibodies
  • Description
    Rabbit monoclonal [EP597Y] to DNAJC3
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human DNAJC3.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
    • Tissue specificity
      Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
    • Sequence similarities
      Contains 1 J domain.
      Contains 9 TPR repeats.
    • Domain
      The J domain mediates interaction with HSPA8.
    • Cellular localization
      Endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • DnaJ (Hsp40) homolog subfamily C member 3 antibody
      • DnaJ homolog subfamily C member 3 antibody
      • DNAJC3 antibody
      • DNJC3_HUMAN antibody
      • double-stranded RNA-activated protein kinase inhibitor antibody
      • Endoplasmic reticulum DnaJ protein 6 antibody
      • ERdj6 antibody
      • HP58 antibody
      • Interferon induced, double stranded RNA activated protein kinase inhibitor antibody
      • Interferon-induced antibody
      • P58 antibody
      • p58 ipk antibody
      • P58IPK antibody
      • PRKRI antibody
      • Protein kinase inhibitor of 58 kDa antibody
      • Protein kinase inhibitor p58 antibody
      • Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody
      see all

    Images

    • Anti-DNAJC3 antibody [EP597Y] (ab52899) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)

    References

    ab52899 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab52899.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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