The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
RelevanceDnaJ is a testis-specific heat shock protein and functions in association with DnaK(Hsp70). It is a molecular chaperone to facilitate protein folding. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70.