The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa). This antibody did not give bands in initial tests on lysate/cell line, we would welcome your feedback if you have tested this antibody in more suitable positive controls
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab156690 (5µg/ml) staining of paraffin embedded Human Testis following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining primarily in spermatogonia and in cellular membrane.