Anti-DNA/RNA Damage antibody [15A3] (ab62623)

Overview

  • Product name
    Anti-DNA/RNA Damage antibody [15A3]
    See all DNA/RNA Damage primary antibodies
  • Description
    Mouse monoclonal [15A3] to DNA/RNA Damage
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to DNA/RNA Damage. 8-hydroxy-guanosine-BSA and -casein conjugates.

  • General notes

    Please see the protocol booklet link below for recommended IHC and ICC staining procedure

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    15A3
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 10 µg/ml.

Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum.

ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

PubMed 25479606

IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21824519

Target

  • Relevance
    In intact animals, lesions (adducts) excised from DNA are transported from the cell through the circulation and excreted in urine. In bacteria, DNA adducts are excreted directly into the medium. In either case, the adducts can be assayed as a measure of oxidative damage to DNA. In particular, Oxo-8-dG (8-Oxo-7,8-dihydro-2'-deoxyguanosine) serves as an excellent marker for DNA damage produced by oxidants because it represents one of the major products generated by a wide array of treatments associated with oxidant damage such as that produced by irradiation and various carcinogens and because it is implicated in spontaneous transversion mutagenesis. Oxo-8-Gua (8-oxo-7,8-dihydroguanine) is one of the most common DNA lesions resulting from reactive oxygen species and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome. In humans, it is primarily repaired by DNA glycosylase OGG1. It can be caused by ionizing radiation, in connection with oxidative metabolism. Oxo-8-G (8-oxo-7,8-dihydroguanosine) is classified as an oxidized ribonucleotide, and is primarily used in studies of oxidative RNA damage and associated RNA repair and RNA turnover mechanisms within the cell. In the cell, Oxo-8-G RNA lesions are formed by reaction with reactive oxygen species (ROS) generated either via normal oxidative metabolic processes, UV ionizing radiation, or exposure to oxidative agents. Oxidative RNA damage can lead to defects in protein synthesis, for example, decreased rates of protein synthesis and production of aggregated or truncated peptides, with important implications in aging and neurodegenerative disorders and artherosclerosis.
  • Alternative names
    • 7,8-Dihydro-8-oxo-2'-deoxyguanosine antibody
    • 7,8-Dihydro-8-oxodeoxyguanosine antibody
    • 7,8-Dihydro-8-oxoguanine antibody
    • 8-Hydroxy-2'-deoxyguanosine antibody
    • 8-Hydroxydeoxyguanosine antibody
    • 8-hydroxyguanine antibody
    • 8-OH-dG antibody
    • 8-Oxo-2'-deoxyguanosine antibody
    • 8-Oxo-7,8-dihydro-2'-deoxyguanosine (Oxo-8-dG) antibody
    • 8-Oxo-7,8-dihydrodeoxyguanosine antibody
    • 8-oxo-7,8-dihydroguanine (Oxo-8-Gua) antibody
    • 8-oxo-7,8-dihydroguanosine (Oxo-8-G) antibody
    • 8-Oxo-dG antibody
    • 8-Oxo-G antibody
    • 8-oxo-Gua antibody
    • 8-Oxo-guanosine antibody
    • 8-Oxo-riboguanosine antibody
    • 8-Oxoguanine antibody
    • OH8Gua antibody
    see all

Images

  • Immunohistochemical analysis of murine brain tissue 24 hours after recirculation following ischemia. Staining using ab62623 at 1/1000 dilution. An AlexaFluor®488-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.

  • Left panel: ab62623 staining in ischemic rat brain tissue
    Centre panel: DAPI staining
    Right panel: merged

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.

    See Abreview

  • ab62623 staining in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.

    See Abreview

References

This product has been referenced in:
  • Prakash Bangalore M  et al. Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells. Sci Rep 7:42222 (2017). Read more (PubMed: 28176872) »
  • Patitucci TN & Ebert AD SMN deficiency does not induce oxidative stress in SMA iPSC-derived astrocytes or motor neurons. Hum Mol Genet 25:514-23 (2016). Read more (PubMed: 26643950) »

See all 28 Publications for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids
Username

Abcam user community

Verified customer

Submitted Apr 11 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100 in TBS for 5-10 minutes
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 09 2016

Application
Immunocytochemistry
Sample
Oncorhynchus mykiss Cell (Spermatozoa)
Permeabilization
Yes - 0,3%Triton-X100 in PBS1X
Specification
Spermatozoa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 06 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Specification
Bone tissue (Femur)
Blocking step
Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mr. Helder Fonseca

Verified customer

Submitted Sep 11 2015

Application
ELISA
Sample
Human Cell (immortalized proximal tubule HK2 cells)
Specification
immortalized proximal tubule HK2 cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 24°C
Type
Other
Username

Abcam user community

Verified customer

Submitted Jul 22 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Cell (Hepatocyte)
Specification
Hepatocyte
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 23 2014

We added IHC of paraffin-embedded tissue to the applications section of the ab62623 datasheet based on reviews we received from customers. These are linked on the main online datasheet. Each customer used a different method for antigen retrieval. One u...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16F10 melanoma)
Specification
B16F10 melanoma
Fixative
4% Formalin
Permeabilization
Yes - 0.1% SDS
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Mar 15 2013

Thank you for contacting us.

Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

Regarding ab23722, human colon ...

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Thank you for your reply.

Could you please confirm the order number so I can raise the new free order for ab62623?

We appreciate any feedback from customers using our products. The suggested tips aimed to help you solve the problems...

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1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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