Anti-DNA/RNA Damage antibody [15A3] (ab62623)
Key features and details
- Mouse monoclonal [15A3] to DNA/RNA Damage
- Suitable for: IHC-P, ICC, ELISA, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-DNA/RNA Damage antibody [15A3]
See all DNA/RNA Damage primary antibodies -
Description
Mouse monoclonal [15A3] to DNA/RNA Damage -
Host species
Mouse -
Specificity
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
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Tested applications
Suitable for: IHC-P, ICC, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to DNA/RNA Damage. 8-hydroxy-guanosine-BSA and -casein conjugates.
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Positive control
- IHC-P: Mouse inflammed colon and backskin tissues. Fresh IHC: Ischemic rat brain tissue.
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General notes
Please see the protocol booklet link below for recommended IHC and ICC staining procedure
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
15A3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
Use a concentration of 1 - 10 µg/ml.
Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum. |
ICC | (1) |
Use at an assay dependent concentration.
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ELISA | (2) |
Use at an assay dependent concentration.
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AP |
Use at an assay dependent concentration.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use a concentration of 1 - 10 µg/ml. Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum. |
ICC
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
AP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
In intact animals, lesions (adducts) excised from DNA are transported from the cell through the circulation and excreted in urine. In bacteria, DNA adducts are excreted directly into the medium. In either case, the adducts can be assayed as a measure of oxidative damage to DNA. In particular, Oxo-8-dG (8-Oxo-7,8-dihydro-2'-deoxyguanosine) serves as an excellent marker for DNA damage produced by oxidants because it represents one of the major products generated by a wide array of treatments associated with oxidant damage such as that produced by irradiation and various carcinogens and because it is implicated in spontaneous transversion mutagenesis. Oxo-8-Gua (8-oxo-7,8-dihydroguanine) is one of the most common DNA lesions resulting from reactive oxygen species and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome. In humans, it is primarily repaired by DNA glycosylase OGG1. It can be caused by ionizing radiation, in connection with oxidative metabolism. Oxo-8-G (8-oxo-7,8-dihydroguanosine) is classified as an oxidized ribonucleotide, and is primarily used in studies of oxidative RNA damage and associated RNA repair and RNA turnover mechanisms within the cell. In the cell, Oxo-8-G RNA lesions are formed by reaction with reactive oxygen species (ROS) generated either via normal oxidative metabolic processes, UV ionizing radiation, or exposure to oxidative agents. Oxidative RNA damage can lead to defects in protein synthesis, for example, decreased rates of protein synthesis and production of aggregated or truncated peptides, with important implications in aging and neurodegenerative disorders and artherosclerosis. -
Alternative names
- 7,8-Dihydro-8-oxo-2'-deoxyguanosine antibody
- 7,8-Dihydro-8-oxodeoxyguanosine antibody
- 7,8-Dihydro-8-oxoguanine antibody
see all
Images
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Bouin’s Fixative, paraffin-embedded mouse backskin tissue stained for DNA/RNA Damage using ab62623 (1 hr at RT) at 1/100 dilution in immunohistochemical analysis.
Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 for 1 hour at RT.
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Mouse hepatocytes stained for DNA/RNA Damage (green) using ab62623 at 1/500 dilution in ICC/IF, followed by Alexa-Fluor®488 conjugated Goat Anti-Mouse IgG (H+L).
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Left panel: ab62623 (1/1000 for 16 hours at RT) staining in ischemic rat brain tissue (fresh samples).
Center panel: DAPI staining
Right panel: mergedSecondary Antibody: Alexa Fluor®546 Goat Anti-mouse (Red) at 1/500 for 1 hour at RT.
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Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labeling with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A secondary was not used, but ab93695 detection kit was used for signal amplification.
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ab62623 staining in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.
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Paraffin-embedded mouse inflammed colon tissue stained for DNA/RNA Damage using ab62623 at 1/1,000,000 dilution (12 hrs at 4°C) in immunohistochemical analysis.
Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (143)
ab62623 has been referenced in 143 publications.
- Yu S et al. BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model. Oxid Med Cell Longev 2022:4054938 (2022). PubMed: 35140838
- Kim J et al. Suppressive Effect of Autocrine FGF21 on Autophagy-Deficient Hepatic Tumorigenesis. Front Oncol 12:832804 (2022). PubMed: 35321438
- Jung KI et al. Neuroprotective Effects of Nicotinamide (Vitamin B3) on Neurodegeneration in Diabetic Rat Retinas. Nutrients 14:N/A (2022). PubMed: 35334819
- Chen H et al. Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. Clin Transl Med 12:e574 (2022). PubMed: 35390228
- Li X et al. Nrf2 Activation Mediates Antiallodynic Effect of Electroacupuncture on a Rat Model of Complex Regional Pain Syndrome Type-I through Reducing Local Oxidative Stress and Inflammation. Oxid Med Cell Longev 2022:8035109 (2022). PubMed: 35498128