Overview

  • Product name
  • Description
    Rabbit polyclonal to DNase I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human DNase I protein.

  • Positive control
    • Human Brain cerebellum and Tonsil tissues; MOLT4 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • Tissue specificity
    Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • Sequence similarities
    Belongs to the DNase I family.
  • Cellular localization
    Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deoxyribonuclease 1 antibody
    • Deoxyribonuclease I antibody
    • Deoxyribonuclease-1 antibody
    • Deoxyribonuclease1 antibody
    • DeoxyribonucleaseI antibody
    • DNAS1_HUMAN antibody
    • DNASE 1 antibody
    • DNase I antibody
    • DNase I lysosomal antibody
    • DNASE1 antibody
    • DNaseI antibody
    • DNL 1 antibody
    • DNL1 antibody
    • Dornase alfa antibody
    • DRNI antibody
    • FLJ38093 antibody
    • Human urine deoxyribonuclease I antibody
    see all

Images

  • ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
  • ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • Anti-DNase I antibody (ab113241) at 1/1000 dilution + MOLT4 cell lysate at 30 µg

    Predicted band size: 31 kDa



    10% SDS PAGE

References

This product has been referenced in:
  • Thiyagarajan D  et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). Read more (PubMed: 29468159) »
  • Fismen S  et al. Impact of the Tumor Necrosis Factor Receptor-Associated Protein 1 (Trap1) on Renal DNaseI Shutdown and on Progression of Murine and Human Lupus Nephritis. Am J Pathol 182:688-700 (2013). Read more (PubMed: 23273922) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
ELISA
Sample
Human Recombinant protein (rhDnase)
Specification
rhDnase
Type
Indirect
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Maria Machado

Verified customer

Submitted Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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