SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].
Tested applicationsSuitable for:
ELISA, Dot blot, WB, Electron Microscopymore details
Deoxyribonuclease I from Bovine Pancreas
Storage instructionsShipped at 4°C. Store at +4°C.
Purification notesPurified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography, followed by extensive dialysis with the storage buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/500 - 1/2000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAmong other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
Tissue specificityPrincipally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
Sequence similaritiesBelongs to the DNase I family.
Cellular localizationSecreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.