Anti-DNase II antibody (ab115233)

Overview

  • Product nameAnti-DNase II antibody
    See all DNase II primary antibodies
  • Description
    Rabbit polyclonal to DNase II
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CNGMARKOSRAYKI conjugated to KLH, corresponding to C terminal amino acids 347-360 of Human DNase II.

  • Positive control
    • Human thymus and brain cerebellum tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
  • Sequence similaritiesBelongs to the DNase II family.
  • Post-translational
    modifications
    Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid DNase antibody
    • Deoxyribonuclease 2 alpha antibody
    • Deoxyribonuclease 2 antibody
    • Deoxyribonuclease II alpha antibody
    • Deoxyribonuclease II antibody
    • Deoxyribonuclease II lysosomal antibody
    • Deoxyribonuclease-2-alpha antibody
    • Deoxyribonuclease2 antibody
    • DeoxyribonucleaseII antibody
    • DNASE 2 antibody
    • DNASE 2A antibody
    • DNase II alpha antibody
    • DNase II lysosomal antibody
    • DNASE2 antibody
    • DNASE2A antibody
    • DNaseII antibody
    • DNL 2 antibody
    • DNL antibody
    • DNL2 antibody
    • DNS2A_HUMAN antibody
    • Lysosomal DNase II antibody
    • R31240 2 antibody
    • R31240_2 antibody
    see all

Anti-DNase II antibody images

  • Human thymus tissue (formalin-fixed, paraffin-embedded) stained with ab115233 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
  • Human cerebellum tissue (formalin-fixed, paraffin-embedded) stained with ab115233 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

References for Anti-DNase II antibody (ab115233)

ab115233 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. These antibodies are almost identical and so either would suit your needs. The only difference I can see is that ab8119 does not recognize the 12 kD beta chain, while it is not specified for ab115233. Currently ab115233 ...

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