Overview

  • Product nameAnti-DNase II antibody
    See all DNase II primary antibodies
  • Description
    Rabbit polyclonal to DNase II
  • SpecificitySpecific for the 32 kD alpha chain; does not recognise the 12 kD beta chain.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Peptide corresponding to amino acids 347 to 360 of human DNase II precursor.

  • Positive control
    • Human spleen tissue lysate
  • General notes


    Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNAfragmentation (Zamzami & Kroemer, 1999). The genes encoding human (Yasuda et al.1998, Shiokawa & Tanuma, 1998, Baker et al, 1998), porcine, and murine DNase II have been cloned (Krieser & Eastman, 1998). The DNaseII gene encodes a 40 kDa proenzyme. The mature enzymeconsists of two non-identical subunits, the 32 kDa (a) and 12kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (Shiokawa & Tanuma, 1998). DNase II is ubiquitously expressed in human tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesApoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNAfragmentation (Zamzami & Kroemer, 1999). The genes encoding human (Yasuda et al.1998, Shiokawa & Tanuma, 1998, Baker et al, 1998), porcine, and murine DNase II have been cloned (Krieser & Eastman, 1998). The DNaseII gene encodes a 40 kDa proenzyme. The mature enzymeconsists of two non-identical subunits, the 32 kDa (a) and 12kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (Shiokawa & Tanuma, 1998). DNase II is ubiquitously expressed in human tissues.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 (precursor) kDa).Can be blocked with DNase II peptide (ab8373).

Target

  • FunctionHydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
  • Sequence similaritiesBelongs to the DNase II family.
  • Post-translational
    modifications
    Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid DNase antibody
    • Deoxyribonuclease 2 alpha antibody
    • Deoxyribonuclease 2 antibody
    • Deoxyribonuclease II alpha antibody
    • Deoxyribonuclease II antibody
    • Deoxyribonuclease II lysosomal antibody
    • Deoxyribonuclease-2-alpha antibody
    • Deoxyribonuclease2 antibody
    • DeoxyribonucleaseII antibody
    • DNASE 2 antibody
    • DNASE 2A antibody
    • DNase II alpha antibody
    • DNase II lysosomal antibody
    • DNASE2 antibody
    • DNASE2A antibody
    • DNaseII antibody
    • DNL 2 antibody
    • DNL antibody
    • DNL2 antibody
    • DNS2A_HUMAN antibody
    • Lysosomal DNase II antibody
    • R31240 2 antibody
    • R31240_2 antibody
    see all

Anti-DNase II antibody images

  • All lanes : Anti-DNase II antibody (ab8119) at 1/500 dilution

    Lane 1 : Human spleen tissue lysate with absence of blocking peptide
    Lane 2 : Human spleen tissue lysate with presence of blocking peptide


    Predicted band size : 36 (precursor) kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab8119 at 5µg/ml staining DNase II in human spleen tissue by IHC

  • ICC/IF image of ab8119 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8119, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DNase II antibody (ab8119)

This product has been referenced in:
  • Robinson MS  et al. Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria. Dev Cell 18:324-31 (2010). WB ; Human . Read more (PubMed: 20159602) »

See 1 Publication for this product

Product Wall

Thank you for contacting us and your interest in our products.

As discussed over the phone, the anti-DNase II antibody (ab8119) has to our knowledge not been used with mouse samples. If we have conclusive information to show that a particular...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for contacting Abcam. These antibodies are almost identical and so either would suit your needs. The only difference I can see is that ab8119 does not recognize the 12 kD beta chain, while it is not specified for ab115233. Currently ab115233 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"