Overview

  • Product nameAnti-DNASE2B antibody
    See all DNASE2B primary antibodies
  • Description
    Rabbit polyclonal to DNASE2B
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: WMAQRLKTHL LTETWQRKRQ ELPSNCSLPY HVYNIKAIKL SRHSYFSSYQ, corresponding to amino acids 254-303 of Human DNASE2B

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 19, 34 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

Target

  • RelevanceDNASE2B shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this protein is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene.
  • Cellular localizationLysosome
  • Database links
  • Alternative names
    • Deoxyribonuclease 2 beta antibody
    • Deoxyribonuclease 2 beta precursor antibody
    • Deoxyribonuclease II beta antibody
    • DLAD antibody
    • DNase 2 like acid DNase antibody
    • DNASE 2B antibody
    • DNase II beta antibody
    • DNase II like acid DNase antibody
    • DNASE2 antibody
    • DNase2 like acid DNase antibody
    • DnaseIIb antibody
    • EC 3.1.22.1 antibody
    • Endonuclease DLAD antibody
    • Lysosomal DNase II antibody
    • UOX antibody
    see all

Anti-DNASE2B antibody images

  • Anti-DNASE2B antibody (ab66738) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa,~70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DNASE2B antibody (ab66738)

ab66738 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"