Overview

  • Product nameAnti-Dnd1 antibody
    See all Dnd1 primary antibodies
  • Description
    Mouse monoclonal to Dnd1
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein tagged with GST (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab57770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells (By similarity). Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs. Sequence-specific RNA-binding factor that binds specifically to U-rich regions (URRs) in the 3' untranslated region (3'-UTR) of several mRNAs. Does not bind to miRNAs. May play a role during primordial germ cell (PGC) survival (By similarity). However, does not seem to be essential for PGC migration.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Cellular localizationNucleus. Cytoplasm. Perinuclear germ granules, also called germ plasm or chromatoid body. Colocalizes in perinuclear sites with APOBEC3.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dead end homolog 1 (zebrafish) antibody
    • dead end homolog 1 antibody
    • Dead end protein antibody
    • Dead end protein homolog 1 antibody
    • Dead end, zebrafish, homolog, 1 antibody
    • DND microRNA-mediated repression inhibitor 1 antibody
    • dnd1 antibody
    • DND1_HUMAN antibody
    • MGC34750 antibody
    • RBMS4 antibody
    • RNA binding motif single stranded interacting protein 4 antibody
    • RNA-binding motif antibody
    • single-stranded-interacting protein 4 antibody
    • Ter antibody
    see all

Anti-Dnd1 antibody images

  • Dnd1 antibody (ab57770) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57770 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57770, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-Dnd1 antibody (ab57770) + PC12
  • Anti-Dnd1 antibody (ab57770) + Raw 264.7
  • Anti-Dnd1 antibody (ab57770) + NIH/3T3

References for Anti-Dnd1 antibody (ab57770)

ab57770 has not yet been referenced specifically in any publications.

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