Overview

  • Product name
    Anti-DNHD1 antibody
  • Description
    Rabbit polyclonal to DNHD1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: KHSQATQPML ILLPPPGHPS ATLHPLTVIQ KLAAKYQQGQ KQLQVIALGS EAWDPVSVVV STLSQAMYEG HWLVLDNCHL MPHW, corresponding to internal sequence amino acids 4080-4163 of Human DNHD1.

  • Positive control
    • Human lung tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Belongs to the dynein heavy chain family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf47 antibody
    • CCDC35 antibody
    • coiled-coil domain containing 35 antibody
    • DHCD1 antibody
    • DNHD1 antibody
    • DNHD1 variant protein antibody
    • DNHD1_HUMAN antibody
    • DNHD1L antibody
    • Dynein heavy chain domain 1-like protein antibody
    • Dynein heavy chain domain-containing protein 1 antibody
    • FLJ32752 antibody
    • FLJ35709 antibody
    • FLJ46184 antibody
    • Protein CCDC35 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab122574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122574, at 1/50 dilution, staining DNHD1 in paraffin-embedded Human lung tissue by Immunohistochemistry.

References

ab122574 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122574.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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