Overview

  • Product name
  • Description
    Mouse polyclonal to DNMBP
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Xenopus laevis
  • Immunogen

    Full length human DNMBP protein (AAH41628.1).

  • Positive control
    • Hela cell lysate; DNMBP transfected 293T cell lysate Hela cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa for isoform 1, and 93 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
    • Tissue specificity
      Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.
    • Cellular localization
      Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    Images

    • Anti-DNMBP antibody (ab88534) at 1 µg/ml + Hela cell lysate at 50 µg

      Predicted band size : 177 , 93 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DNMBP antibody (ab88534) at 1 µg/ml

      Lane 1 : DNMBP transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 177 , 93 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    • ab88534, at 10µg/ml, staining DNMBP in Hela cell by Immunofluorescence.

    References

    ab88534 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    500 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Stage 11 and Stage 32 Embryos)
    Specification
    Stage 11 and Stage 32 Embryos
    Blocking step
    KPL Blocking Reagent as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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