Overview

  • Product name
  • Description
    Mouse polyclonal to DNMBP
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Xenopus laevis
  • Immunogen

    Full length human DNMBP protein (AAH41628.1).

  • Positive control
    • Hela cell lysate; DNMBP transfected 293T cell lysate Hela cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa for isoform 1, and 93 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
    • Tissue specificity
      Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.
    • Cellular localization
      Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    Anti-DNMBP antibody images

    • Anti-DNMBP antibody (ab88534) at 1 µg/ml + Hela cell lysate at 50 µg

      Predicted band size : 177 , 93 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DNMBP antibody (ab88534) at 1 µg/ml

      Lane 1 : DNMBP transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 177 , 93 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    • ab88534, at 10µg/ml, staining DNMBP in Hela cell by Immunofluorescence.

    References for Anti-DNMBP antibody (ab88534)

    ab88534 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Loading amount
    500 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Stage 11 and Stage 32 Embryos)
    Specification
    Stage 11 and Stage 32 Embryos
    Blocking step
    KPL Blocking Reagent as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up