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DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Colorimetric) allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Adomet, 50X||1 x 60µl||1 x 120µl|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Detection Antibody, 400 µg/mL||1 x 6µl||1 x 12µl|
|Developer Solution||1 x 5ml||1 x 10ml|
|DNMT Assay Buffer||1 x 4ml||1 x 8ml|
|DNMT Enzyme Control, 50 µg/mL||1 x 6µl||1 x 12µl|
|Enhancer Solution||1 x 6µl||1 x 12µl|
|Stop Solution||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab113467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"