DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment..
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Adomet, 50X||1 x 60µl||1 x 120µl|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Detection Antibody, 400 µg/mL||1 x 6µl||1 x 12µl|
|DNMT Assay Buffer||1 x 4ml||1 x 8ml|
|DNMT Enzyme Control, 50 µg/mL||1 x 6µl||1 x 12µl|
|Enhancer Solution||1 x 6µl||1 x 12µl|
|Fluoro Developer||1 x 6µl||1 x 12µl|
|Fluoro Diluter||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 6µl||1 x 12µl|
Our Abpromise guarantee covers the use of ab113468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113468 has not yet been referenced specifically in any publications.