Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)


  • Product nameAnti-Dnmt1 antibody [60B1220.1] - ChIP Grade
    See all Dnmt1 primary antibodies
  • Description
    Mouse monoclonal [60B1220.1] to Dnmt1 - ChIP Grade
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
    Predicted to work with: Sheep, Goat, Cow, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 637-650 of Human Dnmt1 (NP_001370).

  • Positive control
    • Human kidney tissue (IHC-P) Mouse embryonic stem and NIH 3T3 cell lysates (WB)



Our Abpromise guarantee covers the use of ab92453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 183 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 15548683


  • FunctionMethylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • Tissue specificityUbiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • Sequence similaritiesBelongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • DomainThe N-terminal part is required for homodimerization and acts as a regulatory domain.
  • Post-translational
    Sumoylated; sumoylation increases activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all

Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade images

  • All lanes : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

    Lane 1 : 2102EP (human embryonic carcinoma) with absence of blocking peptide
    Lane 2 : 2102EP (human embryonic carcinoma) with blocking peptide
    Lane 3 : Recombinant human Dnmt1 protein with absence of blocking peptide
    Lane 4 : Recombinant human Dnmt1 protein with blocking peptide
    Lane 5 : NIH 3T3 (mouse embryonic fibroblast) with absence of blocking peptide
    Lane 6 : NIH 3T3 (mouse embryonic fibroblast) with blocking peptide
    Lane 7 : D3 (mouse embryonic stem cell) with absence of blocking peptide
    Lane 8 : D3 (mouse embryonic stem cell) with blocking peptide

    Goat anti-Mouse IgG HRP-conjugated secondary antibody

    Predicted band size : 183 kDa
    Observed band size : 183 kDa
    Additional bands at : 220 kDa,35 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Lane 1 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 2 µg/ml
    Lane 2 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 0.2 µg/ml

    Lane 1 : Mouse ES cell lysate
    Lane 2 : Mouse NIH3T3 cell lysate

    Goat anti-Mouse Ig HRP conjugated

    Predicted band size : 183 kDa
  • ab92453, at 2 µg/ml, staining Dnmt1 on formalin fixed, paraffin embedded medullar kidney tissue sections by Immunohistochemistry.
  • Overlay histogram showing HeLa cells stained with ab92453 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92453, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

This product has been referenced in:
  • Dai L  et al. Aberrant levels of histone H3 acetylation induce spermatid anomaly in mouse testis. Histochem Cell Biol 143:209-24 (2015). IHC ; Mouse . Read more (PubMed: 25326673) »
  • Halley-Stott RP  et al. Mitosis gives a brief window of opportunity for a change in gene transcription. PLoS Biol 12:e1001914 (2014). WB, ChIP . Read more (PubMed: 25072650) »

See all 10 Publications for this product

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Thank you for contacting us.

If there is no other protocol then our standard protocols suggested on the product data sheet, the general protocols should be sufficient. However, I contacted the laboratory to get the protocol that was used doing...

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Thank you for contacting us.

I can recommend the following secondary antibodies, which are enzyme conjugated to HRP that would be suitable for the detection of the three primary antibodies (Anti-Dnmt3b antibody [52A1018] - ChIP Grade (ab13604)...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (liver cancer)
Loading amount 15 µg
Specification liver cancer
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 18 2012

Application Western blot
Sample Rat Cell lysate - whole cell (cortical cells 15 DIV)
Loading amount 20 µg
Specification cortical cells 15 DIV
Gel Running Conditions Reduced Denaturing (5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 11 2012

Thank you for contacting Abcam.

We do not have the specific peptide that was used to generate ab92543, however the peptide sequence is available on the website and so you would be able to have one custom synthesized.

Please let me k...

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Thank you for attending our webinar on ChIP last week. We hope you enjoyed it and it was useful for you.

We would also like to thank you for sending your questions, and apologise that we were not able to answer them in the Q&A session.

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Thank you for contacting us. I can confirm that I have logged your inquiry in our system and have contacted the laboratory for their opinion. I will get back to you with the information soon. Many thanks for having patience. Please do not hesit...

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