FunctionMethylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Tissue specificityUbiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Sequence similaritiesBelongs to the C5-methyltransferase family. Contains 2 BAH domains. Contains 1 CXXC-type zinc finger.
DomainThe N-terminal part is required for homodimerization and acts as a regulatory domain.
Samples: Nuclear extract [50 µg/lane for A; 10 µg (input) or 1 mg (IP) for B] from HeLa cells.
A) ab11891 used at the indicated concentrations for WB.
B) DNMT1 was immunoprecipitated with ab11891 then detected with ab11890 used at 0.2 µg/ml for WB.
Detection: Chemiluminescence with an exposure time of 2 seconds.
References for Anti-Dnmt1 antibody (ab11891)
This product has been referenced in:
Soberanes S et al. Particulate matter Air Pollution induces hypermethylation of the p16 promoter Via a mitochondrial ROS-JNK-DNMT1 pathway. Sci Rep2:275 (2012).
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