• Product nameAnti-Dnmt1 antibody
    See all Dnmt1 primary antibodies
  • Description
    Goat polyclonal to Dnmt1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 875-888 of human Dnmt1 (NP_001370.1).

  • Positive control
    • Jurkat cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab77689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 183 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMethylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
    • Tissue specificityUbiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
    • Sequence similaritiesBelongs to the C5-methyltransferase family.
      Contains 2 BAH domains.
      Contains 1 CXXC-type zinc finger.
    • DomainThe N-terminal part is required for homodimerization and acts as a regulatory domain.
    • Post-translational
      Sumoylated; sumoylation increases activity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADCADN antibody
      • AIM antibody
      • CXXC finger protein 9 antibody
      • CXXC-type zinc finger protein 9 antibody
      • CXXC9 antibody
      • DNA (cytosine 5 ) methyltransferase 1 antibody
      • DNA (cytosine-5)-methyltransferase 1 antibody
      • DNA methyltransferase 1 antibody
      • DNA methyltransferase HsaI antibody
      • DNA methyltransferase M.HsaI. antibody
      • DNA MTase antibody
      • DNA MTase HsaI antibody
      • DNMT 1 antibody
      • DNMT antibody
      • Dnmt1 antibody
      • DNMT1_HUMAN antibody
      • Dnmt1o antibody
      • FLJ16293 antibody
      • HSN1E antibody
      • M.HsaI antibody
      • MCMT antibody
      • Met1 antibody
      • MGC104992 antibody
      • mMmul antibody
      • MommeD2 antibody
      see all

    Anti-Dnmt1 antibody images

    • Anti-Dnmt1 antibody (ab77689) at 0.5 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 183 kDa
      Observed band size : 183 kDa

      Exposure time : 1 hour

    References for Anti-Dnmt1 antibody (ab77689)

    ab77689 has not yet been referenced specifically in any publications.

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