Anti-Dnmt1 antibody (ab87654)
Key features and details
- Rabbit polyclonal to Dnmt1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Dnmt1 antibody
See all Dnmt1 primary antibodies -
Description
Rabbit polyclonal to Dnmt1 -
Host species
Rabbit -
Specificity
ab87656 is recommended for DNMT1 detection in WB. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form
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General notes
Useful for analysis of the protein complex containing Dnmt1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Traces of Ammonium sulphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Useful for analysis of the protein complex containing Dnmt1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/5000.
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WB |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa).
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Notes |
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ICC/IF
1/5000. |
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa). |
Target
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Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. -
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger. -
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain. -
Post-translational
modificationsSumoylated; sumoylation increases activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1786 Human
- Entrez Gene: 13433 Mouse
- Omim: 126375 Human
- SwissProt: P26358 Human
- SwissProt: P13864 Mouse
- Unigene: 202672 Human
- Unigene: 128580 Mouse
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Alternative names
- ADCADN antibody
- AIM antibody
- CXXC finger protein 9 antibody
see all
Images
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ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
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All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Tagged-NP 95 + BirA Input
Lane 2 : Tagged-NP 95 + BirA Beads
Lane 3 : BirA Input
Lane 4 : BirA Beads
Predicted band size: 183 kDa
Observed band size: 183 kDa
Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna. -
All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
Lane 4 : BirA (0.1M KCl fraction)
Predicted band size: 183 kDa
Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab87654 has been referenced in 25 publications.
- Uysal F et al. Embryo culture media differentially alter DNA methylating enzymes and global DNA methylation in embryos and oocytes. J Mol Histol 53:63-74 (2022). PubMed: 34741214
- Uysal F et al. DNMT enzymes differentially alter global DNA methylation in a stage-dependent manner during spermatogenesis. Andrologia 54:e14357 (2022). PubMed: 34997784
- Bayerl J et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28:1549-1565.e12 (2021). PubMed: 33915080
- Haggerty C et al. Dnmt1 has de novo activity targeted to transposable elements. Nat Struct Mol Biol 28:594-603 (2021). PubMed: 34140676
- Wang W et al. Mutation-induced DNMT1 cleavage drives neurodegenerative disease. Sci Adv 7:eabe8511 (2021). PubMed: 34516921