Validated using a knockout cell line

Anti-Dnmt1 antibody [EPR3522] (ab92314)


  • Product name
    Anti-Dnmt1 antibody [EPR3522]
    See all Dnmt1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3522] to Dnmt1
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues at the C-terminus of Human Dnmt1.

  • Positive control
    • HuT-78, Jurkat or 293T lysate HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Dnmt1 antibody (Phycoerythrin) [EPR3522] (ab208999)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab92314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 183 kDa.
IP 1/20.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
    • Tissue specificity
      Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
    • Sequence similarities
      Belongs to the C5-methyltransferase family.
      Contains 2 BAH domains.
      Contains 1 CXXC-type zinc finger.
    • Domain
      The N-terminal part is required for homodimerization and acts as a regulatory domain.
    • Post-translational
      Sumoylated; sumoylation increases activity.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • ADCADN antibody
      • AIM antibody
      • CXXC finger protein 9 antibody
      • CXXC-type zinc finger protein 9 antibody
      • CXXC9 antibody
      • DNA (cytosine 5 ) methyltransferase 1 antibody
      • DNA (cytosine-5)-methyltransferase 1 antibody
      • DNA methyltransferase 1 antibody
      • DNA methyltransferase HsaI antibody
      • DNA methyltransferase M.HsaI. antibody
      • DNA MTase antibody
      • DNA MTase HsaI antibody
      • DNMT 1 antibody
      • DNMT antibody
      • Dnmt1 antibody
      • DNMT1_HUMAN antibody
      • Dnmt1o antibody
      • FLJ16293 antibody
      • HSN1E antibody
      • M.HsaI antibody
      • MCMT antibody
      • Met1 antibody
      • MGC104992 antibody
      • mMmul antibody
      • MommeD2 antibody
      see all


    • Predicted band size : 183 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: DNMT1 knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HEK293 whole cell lysate (20 µg)
      Lane 4: HeLa whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92314 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab92314 was shown to specifically react with DNMT1 when DNMT1 knockout samples were used. Wild-type and DNMT1 knockout samples were subjected to SDS-PAGE.  Ab92314 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.


    • Overlay histogram showing HeLa cells stained with ab92314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92314, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Dnmt1 antibody [EPR3522] (ab92314) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 183 kDa
    • Immunofluorescence staining of Jurkat cells with purified ab92314 at a working dilution of 1/2000, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.


    This product has been referenced in:
    • Trejbalová K  et al. Development of 5' LTR DNA methylation of latent HIV-1 provirus in cell line models and in long-term-infected individuals. Clin Epigenetics 8:19 (2016). WB . Read more (PubMed: 26900410) »
    • Clements EG  et al. DNMT1 modulates gene expression without its catalytic activity partially through its interactions with histone-modifying enzymes. Nucleic Acids Res 40:4334-46 (2012). WB . Read more (PubMed: 22278882) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your inquiry.

    I have been in touch with the lab and they can provide custom conjugation of this antibody to biotin. They do require about a 1mg order. Did you have a quantity in mind so that I can get a quote from the lab?

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    Thank you for contacting us. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***. To check the status o...

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    Thank you for contacting us and for sending an image of your staining. Our Abpromise Guarantee covers all of our products for the tested species and applications listed on our datasheet for six months from the date of purchase. In the applicati...

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