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DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.
DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
|Components||96 tests||48 tests|
|10X Wash Buffer||1 x 28ml||1 x 14ml|
|8-Well Assay Strips (with Frame)||12 units||6 units|
|Affinity Antibody, 500 µg/mL||1 x 16µl||1 x 8µl|
|Assay Buffer||1 x 10ml||1 x 5ml|
|Blocking Buffer||1 x 20ml||1 x 10ml|
|Detection Antibody, 200 µg/mL||1 x 20µl||1 x 10µl|
|Developing Solution||1 x 12ml||1 x 6ml|
|DNMT1 Standard, 20 µg/mL||1 x 40µl||1 x 20µl|
|Stop Solution||1 x 11ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113469 has not yet been referenced specifically in any publications.