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DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase. The selective inhibition of DNMT1 may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT1 inhibitors could be a new addition to cancer therapeutic agents.
Abcam's DNMT1 Inhibitor Screening Assay Kit (ab113465) allows the user to selectively screen DNMT1 inhibitors quickly. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 activity/inhibitor screening experiment without the need for radioactivity or any special equipment.
Not sure if this is the right product for you? Check out our DNMT Product Guide for help.
It might be necessary to purchase DNMT1 enzyme - we recommend Recombinant human DNMT1 protein (ab198109).
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 11ml||1 x 22ml|
|8-well Substrate-Coated Strip (with frame)||6 units||12 units|
|Adomet||1 x 35µl||1 x 70µl|
|Capture Antibody||1 x 5µl||1 x 8µl|
|Detection Antibody||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 6ml||1 x 12ml|
|DNMT Assay Buffer||1 x 2ml||1 x 4ml|
|Enhancer Solution||1 x 6µl||1 x 12µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"