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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Protein G purified
Purification notes This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralised immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 55 kDa plus a range of other bands (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
Tissue specificity Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
Sequence similarities Belongs to the C5-methyltransferase family.
Cellular localization Nucleus.
Information by UniProt
DmMT 2 antibody
Anti-Dnmt2 antibody images
Western blot - Dnmt2 antibody (ab71015)
All lanes : Anti-Dnmt2 antibody (ab71015) at 1/100 dilution Lane 1 : Jurkat cell lysate Lane 2 : Mouse liver tissue lysate Lysates/proteins at 12.5 µg per lane. Secondary HRP-anti-rabbit Predicted band size : 45 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dnmt2 antibody (ab71015)
Formalin fixed, paraffin embedded human hepatocarcinoma tissue reacted with ab71015 at a 1/50 dilution to stain Dnmt2. ab71015 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
References for Anti-Dnmt2 antibody (ab71015)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"