Overview

  • Product name
  • Description
    Rabbit polyclonal to Dnmt2
  • Specificity
    This antibody will recognise the splice isoforms A, B, and C.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the human Dnmt2 sequence within amino acids 300~400.

  • Positive control
    • Human hepatocarcinoma tissue, Jurkat cell lysate and mouse liver tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralised immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 55 kDa plus a range of other bands (predicted molecular weight: 45 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
    • Tissue specificity
      Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the C5-methyltransferase family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • dDNMT antibody
      • DmMT 2 antibody
      • DmMT2 antibody
      • DNA (cytosine 5 ) methyltransferase 2 antibody
      • DNA (cytosine 5) methyltransferase like protein 2 antibody
      • DNA (cytosine-5)-methyltransferase-like protein 2 antibody
      • DNA 5 cytosine methyltransferase antibody
      • DNA methyltransferase 2 antibody
      • DNA methyltransferase homolog HsaIIP antibody
      • DNA MTase homolog HsaIIP antibody
      • Dnmt 2 antibody
      • Dnmt2 antibody
      • M.HsaIIP antibody
      • MHsaIIP antibody
      • nmt 2 antibody
      • nmt2 antibody
      • OTTHUMP00000045198 antibody
      • PuMet antibody
      • RNMT 1 antibody
      • RNMT1 antibody
      • TRDMT 1 antibody
      • TRDMT_HUMAN antibody
      • TRDMT1 antibody
      • tRNA (cytosine 5 ) methyltransferase antibody
      • tRNA (cytosine(38)-C(5))-methyltransferase antibody
      • tRNA aspartic acid methyltransferase 1 antibody
      • tRNA aspartic acid methyltransferase 1 variant 1 antibody
      • tRNA aspartic acid methyltransferase 1 variant 2 antibody
      • tRNA aspartic acid methyltransferase 1 variant 3 antibody
      • tRNA aspartic acid methyltransferase 1 variant 4 antibody
      • tRNA aspartic acid methyltransferase 1 variant 5 antibody
      • tRNA aspartic acid methyltransferase 1 variant 8 antibody
      see all

    Images

    • All lanes : Anti-Dnmt2 antibody (ab71015) at 1/100 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Mouse liver tissue lysate

      Lysates/proteins at 12.5 µg per lane.

      Secondary
      HRP-anti-rabbit

      Predicted band size : 45 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Formalin fixed, paraffin embedded human hepatocarcinoma tissue reacted with ab71015 at a 1/50 dilution to stain Dnmt2. ab71015 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

    References

    ab71015 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab71015.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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