Overview

  • Product name
  • Description
    Rabbit polyclonal to Dnmt2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (MILMSPPCQP FTRIGRQGDM TDSRTNSFLH ILDILPRLQK LPKYILLENV) of Human Dnmt2 (NP_004403)

  • Positive control
    • Fetal muscle lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Function
    Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
  • Tissue specificity
    Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the C5-methyltransferase family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • dDNMT antibody
    • DmMT 2 antibody
    • DmMT2 antibody
    • DNA (cytosine 5 ) methyltransferase 2 antibody
    • DNA (cytosine 5) methyltransferase like protein 2 antibody
    • DNA (cytosine-5)-methyltransferase-like protein 2 antibody
    • DNA 5 cytosine methyltransferase antibody
    • DNA methyltransferase 2 antibody
    • DNA methyltransferase homolog HsaIIP antibody
    • DNA MTase homolog HsaIIP antibody
    • Dnmt 2 antibody
    • Dnmt2 antibody
    • M.HsaIIP antibody
    • MHsaIIP antibody
    • nmt 2 antibody
    • nmt2 antibody
    • OTTHUMP00000045198 antibody
    • PuMet antibody
    • RNMT 1 antibody
    • RNMT1 antibody
    • TRDMT 1 antibody
    • TRDMT_HUMAN antibody
    • TRDMT1 antibody
    • tRNA (cytosine 5 ) methyltransferase antibody
    • tRNA (cytosine(38)-C(5))-methyltransferase antibody
    • tRNA aspartic acid methyltransferase 1 antibody
    • tRNA aspartic acid methyltransferase 1 variant 1 antibody
    • tRNA aspartic acid methyltransferase 1 variant 2 antibody
    • tRNA aspartic acid methyltransferase 1 variant 3 antibody
    • tRNA aspartic acid methyltransferase 1 variant 4 antibody
    • tRNA aspartic acid methyltransferase 1 variant 5 antibody
    • tRNA aspartic acid methyltransferase 1 variant 8 antibody
    see all

Images

  • Anti-Dnmt2 antibody (ab82659) at 1 µg/ml (5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References

ab82659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab82659.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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