Anti-Dnmt3b antibody (ab122932)
Key features and details
- Rabbit polyclonal to Dnmt3b
- Suitable for: IHC-Fr, WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3b antibody
See all Dnmt3b primary antibodies -
Description
Rabbit polyclonal to Dnmt3b -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Solubilized mouse embryos at the stages of E7.0-E16.5; transiently transfected 293T cells; E4.5, 5.5 and 7.0 mouse embryo sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), Ammonium sulphate
trace of ammonium sulphate only -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/2000 - 1/5000.
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WB |
1/3000. Predicted molecular weight: 96 kDa.
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ICC/IF |
1/5000.
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Notes |
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IHC-Fr
1/2000 - 1/5000. |
WB
1/3000. Predicted molecular weight: 96 kDa. |
ICC/IF
1/5000. |
Target
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Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. -
Tissue specificity
Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. -
Involvement in disease
Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 13436 Mouse
- SwissProt: O88509 Mouse
- Unigene: 89772 Mouse
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Alternative names
- Cytosine 5methyltransferase 3B antibody
- DNA antibody
- DNA (cytosine 5) methyltransferase 3 beta antibody
see all
Images
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All lanes : Anti-Dnmt3b antibody (ab122932) at 1/3000 dilution
Lane 1 : wild-type mouse embryonic stem cell lysate with 5% skim milk
Lane 2 : Dnmt3b/3a knockout mouse embryonic stem cell lysate with 5% skim milk
Predicted band size: 96 kDa -
Immunocytochemistry/ Immunofluorescence analysis of wild-type (left) and Dnmt3b/3a knockout (right) mouse embryonic stem cells using ab122932 at 1/5000 dilution. DNA was stained with DAPI (bottom). Cell were fixed with 4% PFA/PBS for 30 min at 4℃.
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All lanes : Anti-Dnmt3b antibody (ab122932) at 1/1000 dilution
Lane 1 : stages E7.0 solubilized mouse embryo at 10 µg
Lane 2 : stages E8.5 solubilized mouse embryo at 10 µg
Lane 3 : stages E9.5 solubilized mouse embryo at 10 µg
Lane 4 : stages E10.5 solubilized mouse embryo at 10 µg
Lane 5 : stages E12.5 solubilized mouse embryo at 10 µg
Lane 6 : stages E14.5 solubilized mouse embryo at 10 µg
Lane 7 : stages E16.5 solubilized mouse embryo at 10 µg
Lane 8 : 293T cells transiently transfected with 3b1 isoform of Dnmt3b
Lane 9 : 293T cells transiently transfected with 3b2 isoform of Dnmt3b
Lane 10 : 293T cells transiently transfected with 3b3 isoform of Dnmt3b
Predicted band size: 96 kDa
The embryos were solubilized by sonication or homogenization in the presence of 0.1% SDS. -
Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining using 1/1000 ab122932.
Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with ab122932 or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows and arrowheads, respectively. The epiblast (E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab122932 has been referenced in 15 publications.
- Liang S et al. CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells. Nucleic Acids Res 50:2905-2922 (2022). PubMed: 35212377
- Ding L et al. DNA methylation-independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins. Life Sci Alliance 5:N/A (2022). PubMed: 35288457
- Szigeti KA et al. Global DNA hypomethylation of colorectal tumours detected in tissue and liquid biopsies may be related to decreased methyl-donor content. BMC Cancer 22:605 (2022). PubMed: 35655145
- Xu L et al. Abnormal neocortex arealization and Sotos-like syndrome-associated behavior in Setd2 mutant mice. Sci Adv 7:N/A (2021). PubMed: 33523829
- Wang L et al. Methylation of the miR-29b-3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer. Oncol Rep 45:65-72 (2021). PubMed: 33416135