The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 96 kDa.
FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Tissue specificityUbiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Involvement in diseaseDefects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
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Anti-Dnmt3b antibody - ChIP Grade images
Western blot - Anti-Dnmt3b antibody - ChIP Grade (ab122932)
All lanes : Anti-Dnmt3b antibody - ChIP Grade (ab122932) at 1/1000 dilution
Lane 1 : stages E7.0 solubilized mouse embryo at 10 µg Lane 2 : stages E8.5 solubilized mouse embryo at 10 µg Lane 3 : stages E9.5 solubilized mouse embryo at 10 µg Lane 4 : stages E10.5 solubilized mouse embryo at 10 µg Lane 5 : stages E12.5 solubilized mouse embryo at 10 µg Lane 6 : stages E14.5 solubilized mouse embryo at 10 µg Lane 7 : stages E16.5 solubilized mouse embryo at 10 µg Lane 8 : 293T cells transiently transfected with 3b1 isoform of Dnmt3b Lane 9 : 293T cells transiently transfected with 3b2 isoform of Dnmt3b Lane 10 : 293T cells transiently transfected with 3b3 isoform of Dnmt3b
Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining using 1/1000 ab122932.
Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with ab122932 or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows and arrowheads, respectively. The epiblast (E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.
References for Anti-Dnmt3b antibody - ChIP Grade (ab122932)
This product has been referenced in:
Ip J et al. Normal DNA methylation dynamics in DICER1-deficient mouse embryonic stem cells. PLoS Genet8:e1002919 (2012).
Read more (PubMed: 22969435) »
Sakai Y et al. Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos. Gene Expr Patterns5:231-7 (2004).
Read more (PubMed: 15567719) »