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Products:Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs
Overview
Product name
Anti-Dnmt3b antibody - ChIP Grade
See all Dnmt3b products (9) ...
Description
Rabbit polyclonal to Dnmt3b - ChIP Grade
Specificity
This antibody detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This antibody detects, to a lesser extent, full-length human recombinant Dnmt3a.
Tested applications
ICC/IF, IP, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: MKGDSRHLNEEEGA-C, corresponding to amino acids 1-14 of Mouse Dnmt3b.
MKGDSRHLNE EEGA-C
Positive control
P19 nuclear extracts. ChIP: PMID: 16357870 (Vire E et al Nature 2006 439:871) has used U2OS cells and primers specific for the hsMYT1 promoter. The primers are described in PMID: 15231737 (Kirmizis A et al Genes and Dev 2004 18:1592).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IP: Use at an assay dependent dilution. (PubMed: 16631596)
WB: Use a concentration of 2.0 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 97.5 kDa).
ChIP: Use at an assay dependent dilution.
Target
Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Tissue specificity
Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Involvement in disease
Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBC3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytosine 5methyltransferase 3B antibody
- DNA (cytosine 5) methyltransferase 3 beta antibody
- DNA (cytosine 5)-methyltransferase 3B antibody
see all
Database links
- Entrez Gene: 1789 Human
- Entrez Gene: 13436 Mouse
- Entrez Gene: 444985 Rat
- Omim: 602900 Human
- SwissProt: Q9UBC3 Human
- SwissProt: O88509 Mouse
- Unigene: 643024 Human
- Unigene: 713611 Human
see all
Anti-Dnmt3b antibody - ChIP Grade images:
Immunocytochemistry/ Immunofluorescence-Dnmt3b antibody - ChIP Grade(ab2851)
ICC/IF image of ab2851 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2851, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Dnmt3b antibody - ChIP Grade (ab2851)
All lanes : Anti-Dnmt3b antibody - ChIP Grade (ab2851) at 1 µg/ml
Lane 1 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 97.5 kDa
Observed band size : 97.5 kDa
Additional bands at : 32 kDa,46 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
References for Anti-Dnmt3b antibody - ChIP Grade (ab2851)
This product has been referenced in:
- Angrisano Tet al. Chromatin and DNA methylation dynamics during retinoic acid-induced RET gene transcriptional activation in neuroblastoma cells. Nucleic Acids Res 39:1993-2006 (2011). ChIP; Human.Read more (PubMed: 20952403) »
- Leonard Set al. Epigenetic and Transcriptional Changes Which Follow Epstein-Barr Virus Infection of Germinal Center B Cells and Their Relevance to the Pathogenesis of Hodgkin's Lymphoma. J Virol 85:9568-77 (2011).Read more (PubMed: 21752916) »
See all 14 publications for this product
Publishing research using ab2851? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"