Overview

  • Product nameAnti-Dnmt3b antibody [EPR3523]
    See all Dnmt3b primary antibodies
  • Description
    Rabbit monoclonal [EPR3523] to Dnmt3b
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in human Dnmt3b.

  • Positive control
    • A549, HeLa and A431 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR3523
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 96 kDa.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
    • Tissue specificityUbiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
    • Involvement in diseaseDefects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
    • Sequence similaritiesBelongs to the C5-methyltransferase family.
      Contains 1 ADD domain.
      Contains 1 GATA-type zinc finger.
      Contains 1 PHD-type zinc finger.
      Contains 1 PWWP domain.
    • DomainThe PWWP domain is essential for targeting to pericentric heterochromatin.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytosine 5methyltransferase 3B antibody
      • DNA antibody
      • DNA (cytosine 5) methyltransferase 3 beta antibody
      • DNA (cytosine 5)-methyltransferase 3B antibody
      • DNA (cytosine-5)-methyltransferase 3B antibody
      • DNA methyltransferase HsaIIIB antibody
      • DNA MTase HsaIIIB antibody
      • DNM3B_HUMAN antibody
      • Dnmt3b antibody
      • EC 2.1.1.37 antibody
      • ICF antibody
      • ICF1 antibody
      • M.HsaIIIB antibody
      • MGC124407 antibody
      • RP23-89H14.3 antibody
      see all

    Anti-Dnmt3b antibody [EPR3523] images

    • All lanes : Anti-Dnmt3b antibody [EPR3523] (ab79822) at 1/1000 dilution

      Lane 1 : Flag-tagged Dnmt3b
      Lane 2 : Dnmt3b knockout mouse embryonic stem cell extract
      Lane 3 : Wild type mouse embryonic stem cell extract
      Lane 4 : Wild type mouse embryonic stem cell extract

      Lysates/proteins at 40 µg per lane.

      Secondary
      Donkey anti-rabbit IgG conjugated to HRP (NA934) at 1/1000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 96 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      This image is courtesy of an anonymous abreview.

      Blocking: 5% Milk

      See Abreview

    • All lanes : Anti-Dnmt3b antibody [EPR3523] (ab79822) at 1/5000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 96 kDa
      Observed band size : 96 kDa

    References for Anti-Dnmt3b antibody [EPR3523] (ab79822)

    This product has been referenced in:
    • Yan H  et al. piRNA-823 contributes to tumorigenesis by regulating de novo DNA methylation and angiogenesis in multiple myeloma. Leukemia N/A:N/A (2014). Read more (PubMed: 24732595) »
    • Pan K  et al. HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence. Mol Cell Biol 33:887-903 (2013). Read more (PubMed: 23249948) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Testis)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Testis
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Testis)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Testis
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Wako target retrieval solution
    Sample Marmoset (common) Tissue sections (adult testis)
    Specification adult testis
    Permeabilization No
    Fixative Formaldehyde
    Username

    煜欽 林

    Verified customer

    Submitted Dec 18 2013

    Abreviews
    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (8 % sds-page)
    Sample Mouse Cell lysate - nuclear (mouse embryonic stem cells)
    Specification mouse embryonic stem cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 24 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"