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DNMT3B (DNA (cytosine-5)-methyltransferase 3B) belongs to the DNMT family, it is required for genome de novo methylation and it is essential for establishment of DNA methylation patterns during development. The selective inhibition of DNMT3B may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT3B inhibitors could be a new addition to cancer therapeutic agents.
Abcam's DNMT3B Inhibitor Screening Assay Kit (ab113466) allows the user to selectively screen DNMT3B activity/inhibitors in an ELISA-like format. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B activity/inhibitor screening experiment without the need for radioactivity or any special equipment.
Not sure if this is the right product for you? Check out our DNMT Product Guide for help.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 11ml||1 x 22ml|
|8-well Substrate-Coated Strip (with frame)||6 units||12 units|
|Adomet, 8 mM||1 x 35µl||1 x 70µl|
|Capture Antibody||1 x 5µl||1 x 10µl|
|Detection Antibody||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 6ml||1 x 12ml|
|DNMT Assay Buffer||1 x 2ml||1 x 4ml|
|Enhancer Solution||1 x 6µl||1 x 12µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113466 has not yet been referenced specifically in any publications.