The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
FunctionCatalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
Tissue specificityExpressed at low levels in several tissues including testis, ovary, and thymus.
Sequence similaritiesBelongs to the C5-methyltransferase family. Contains 1 ADD-type zinc finger.