Overview

  • Product name
  • Description
    Chicken polyclonal to Dnmt3L
  • Specificity
    ab28968 recognises Dnmt3L.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Dnmt3L.
    Database link: Q9UJW3

  • Positive control
    • Purified recombinant Human Dnmt3L.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purification notes
    ab28968 is a crude IgY preparation from egg yolks using a precipitation method (PEG, Polyethylene Glycol).
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
  • Tissue specificity
    Expressed at low levels in several tissues including testis, ovary, and thymus.
  • Sequence similarities
    Belongs to the C5-methyltransferase family.
    Contains 1 ADD-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosine 5 methyltransferase 3 like protein antibody
    • DNA (cytosine 5 ) methyltransferase 3 like antibody
    • DNA (cytosine-5)-methyltransferase 3-like antibody
    • DNA cytosine 5 methyltransferase 3 like protein antibody
    • DNA methyltransferase 3 like protein antibody
    • DNM3L_HUMAN antibody
    • Dnmt 3L antibody
    • Dnmt3l antibody
    • Human cytosine 5 methyltransferase 3 like protein antibody
    • MGC1090 antibody
    see all

Anti-Dnmt3L antibody images

  • Anti-Dnmt3L antibody (ab28968) at 1/500 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

References for Anti-Dnmt3L antibody (ab28968)

ab28968 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Hela cells)
Loading amount
40 µg
Specification
Hela cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Sep 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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