Anti-Dnmt3L antibody [N117/9] (ab93613)

Overview

  • Product name
    Anti-Dnmt3L antibody [N117/9]
    See all Dnmt3L primary antibodies
  • Description
    Mouse monoclonal [N117/9] to Dnmt3L
  • Specificity
    Detects Dnmt3L, it does not react with DNMT3a or DNMT3B.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-387 of Human Dnmt3L (accession number BAA95556)

  • General notes

    The clone number has been updated from S117-9 to N117/9, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N117/9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
IHC-Fr
  • Application notes
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 44 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
    • Tissue specificity
      Expressed at low levels in several tissues including testis, ovary, and thymus.
    • Sequence similarities
      Belongs to the C5-methyltransferase family.
      Contains 1 ADD-type zinc finger.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytosine 5 methyltransferase 3 like protein antibody
      • DNA (cytosine 5 ) methyltransferase 3 like antibody
      • DNA (cytosine-5)-methyltransferase 3-like antibody
      • DNA cytosine 5 methyltransferase 3 like protein antibody
      • DNA methyltransferase 3 like protein antibody
      • DNM3L_HUMAN antibody
      • Dnmt 3L antibody
      • Dnmt3l antibody
      • Human cytosine 5 methyltransferase 3 like protein antibody
      • MGC1090 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Mouse backskin labeling Dnmt3L with ab93613 at 1 ug/ml

    • Immunofluorescent analysis on Human HaCaT cells labeling Dnmt3L with ab93613 at 1/1000 dilution.



    • Predicted band size : 44 kDa
      Western blot detection of DNMT3L in a Human tissue mix using ab93613 at 1:1000 dilution.

    References

    ab93613 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample
    Marmoset (common) Tissue sections (adult testis)
    Specification
    adult testis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    煜欽 林

    Verified customer

    Submitted Jul 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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