Anti-Dnmt3L antibody [S117-9] (ab93613)

Overview

  • Product nameAnti-Dnmt3L antibody [S117-9]
    See all Dnmt3L primary antibodies
  • Description
    Mouse monoclonal [S117-9] to Dnmt3L
  • SpecificityDetects Dnmt3L, it does not react with DNMT3a or DNMT3B.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-387 of Human Dnmt3L (accession number BAA95556)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberS117-9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
IHC-Fr
  • Application notesICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 44 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
    • Tissue specificityExpressed at low levels in several tissues including testis, ovary, and thymus.
    • Sequence similaritiesBelongs to the C5-methyltransferase family.
      Contains 1 ADD-type zinc finger.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytosine 5 methyltransferase 3 like protein antibody
      • DNA (cytosine 5 ) methyltransferase 3 like antibody
      • DNA (cytosine-5)-methyltransferase 3-like antibody
      • DNA cytosine 5 methyltransferase 3 like protein antibody
      • DNA methyltransferase 3 like protein antibody
      • DNM3L_HUMAN antibody
      • Dnmt 3L antibody
      • Dnmt3l antibody
      • Human cytosine 5 methyltransferase 3 like protein antibody
      • MGC1090 antibody
      see all

    Anti-Dnmt3L antibody [S117-9] images

    • Immunohistochemical analysis of paraffin-embedded Mouse backskin labeling Dnmt3L with ab93613 at 1 ug/ml

    • Immunofluorescent analysis on Human HaCaT cells labeling Dnmt3L with ab93613 at 1/1000 dilution.



    • Predicted band size : 44 kDa
      Western blot detection of DNMT3L in a Human tissue mix using ab93613 at 1:1000 dilution.

    References for Anti-Dnmt3L antibody [S117-9] (ab93613)

    ab93613 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample Marmoset (common) Tissue sections (adult testis)
    Specification adult testis
    Permeabilization No
    Fixative Formaldehyde
    Username

    煜欽 林

    Verified customer

    Submitted Jul 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"