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Tissue/ cell preparation: DO-11.10 T Cell Receptor hybridoma (Mouse).
The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested. The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules. The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.
Our Abpromise guarantee covers the use of ab93556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.125-0.25µg for 105-8 cells.
Use 0.125 - 0.25µg for 105-8 cells in a final volume of 100µl.
ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ab93556 has not yet been referenced specifically in any publications.