Anti-DO-11.10 T Cell Receptor antibody [KJ1-26.1] (ab75152)

Overview

  • Product nameAnti-DO-11.10 T Cell Receptor antibody [KJ1-26.1]
    See all DO-11.10 T Cell Receptor primary antibodies
  • Description
    Mouse monoclonal [KJ1-26.1] to DO-11.10 T Cell Receptor
  • SpecificityThis antibody is specific for the DO-11.10 T cell receptor idiotype. The DO-11.10 TCR is specific for chicken ovalbumin (aa 323-339) in combination with I-Ad MHC class II complex. KJ1-26 was reported to block the antigen response of the DO-11.10 T cell hybridomas in vitro.
  • Tested applicationsSuitable for: Flow Cyt, Inhibition Assay, IP, ELISA, Othermore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: DO-11.10 T cell hybridoma.

  • General notes


    The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThe KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.
  • ClonalityMonoclonal
  • Clone numberKJ1-26.1
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration.

Inhibition of function of DO-11.10 cells.

IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

Cell surface cytofluorography: Use at an assay dependent dilution.

Target

  • RelevanceThe TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules.
  • Cellular localizationPlasma membrane
  • Alternative names
    • DO 11.10 antibody
    • DO-11.10 antibody
    • DO11.10.24 antibody
    • KJ1.26 antibody

References for Anti-DO-11.10 T Cell Receptor antibody [KJ1-26.1] (ab75152)

This product has been referenced in:
  • Haskins K  et al. The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J Exp Med 157:1149-69 (1983). Read more (PubMed: 6601175) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"