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Tissue/ cell preparation: DO-11.10 T cell hybridoma.
The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.
Our Abpromise guarantee covers the use of ab75152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|Inhibition Assay||Use at an assay dependent concentration.
Inhibition of function of DO-11.10 cells.
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Other||Use at an assay dependent concentration.
Cell surface cytofluorography: Use at an assay dependent dilution.