Overview

  • Product name
  • Description
    Rabbit Polyclonal to DOC2A
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human DOC2A aa 277-306 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: NP_003577.2

  • Positive control
    • WB: K562 cell lysate. IHC-P Human brain tissue. Flow Cyt: K562 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab171308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 44 kDa.

Target

  • Function
    Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
  • Tissue specificity
    Predominantly expressed in brain. Also expressed in testis.
  • Sequence similarities
    Contains 2 C2 domains.
  • Domain
    C2 domain 1 is involved in binding calcium and phospholipids.
  • Cellular localization
    Lysosome. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Colocalizes to synaptic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Doc2 antibody
    • Doc2-alpha antibody
    • Doc2a antibody
    • DOC2A_HUMAN antibody
    • Double C2 domain alpha antibody
    • Double C2 like domains, alpha antibody
    • Double C2-like domain-containing protein alpha antibody
    see all

Images

  • Anti-DOC2A antibody (ab171308) + K562 cell lysate at 35 µg

    Predicted band size : 44 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling DOC2A with ab171308. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • Flow cytometric analysis of K562 cells labeling DOC2A (green) using ab171308 compared to a negative control cell (blue). A FITC-conjugated donkey anti-rabbit IgG was used as secondary antibody.

References

ab171308 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171308.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up