Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab1348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot – Approx 150-200kDa band seen in A431 cell lysate.
    Recommended for use at 3-4µg/ml.
  • Target

    • FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
    • Tissue specificityHighly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
    • Sequence similaritiesBelongs to the DOCK family.
      Contains 1 DHR-1 (CZH-1) domain.
      Contains 1 DHR-2 (CZH-2) domain.
      Contains 1 SH3 domain.
    • DomainThe DHR-2 domain is necessary and sufficient for the GEF activity.
    • Cellular localizationCytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
    • Information by UniProt
    • Database links
    • Alternative names
      • 180 kDa protein downstream of CRK antibody
      • Ced5 antibody
      • Dedicator of Cytokinesis 1 antibody
      • Dedicator of cytokinesis protein 1 antibody
      • DOCK 1 antibody
      • DOCK 180 antibody
      • DOCK1 antibody
      • DOCK1_HUMAN antibody
      • DOCK180 antibody
      see all

    Anti-DOCK1 antibody images

    • DOCK1 staining (4µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence

    References for Anti-DOCK1 antibody (ab1348)

    ab1348 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"