Overview

  • Product name
  • Description
    Rabbit polyclonal to DOCK1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1715 and 1765 of human DOCK1 (NP_001371.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
    • Tissue specificity
      Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
    • Sequence similarities
      Belongs to the DOCK family.
      Contains 1 DHR-1 (CZH-1) domain.
      Contains 1 DHR-2 (CZH-2) domain.
      Contains 1 SH3 domain.
    • Domain
      The DHR-2 domain is necessary and sufficient for the GEF activity.
    • Cellular localization
      Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
    • Information by UniProt
    • Database links
    • Alternative names
      • 180 kDa protein downstream of CRK antibody
      • Ced5 antibody
      • Dedicator of Cytokinesis 1 antibody
      • Dedicator of cytokinesis protein 1 antibody
      • DOCK 1 antibody
      • DOCK 180 antibody
      • DOCK1 antibody
      • DOCK1_HUMAN antibody
      • DOCK180 antibody
      see all

    Images

    • Detection of DOCK1 by Western Blot of Immunprecipitate.
      anti-DOCK1 at 1µg/ml staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab75278 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab75278 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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