The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
Tissue specificityHighly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
Sequence similaritiesBelongs to the DOCK family. Contains 1 DHR-1 (CZH-1) domain. Contains 1 DHR-2 (CZH-2) domain. Contains 1 SH3 domain.
DomainThe DHR-2 domain is necessary and sufficient for the GEF activity.
Cellular localizationCytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
Detection of DOCK1 by Western Blot of Immunprecipitate.
anti-DOCK1 at 1µg/ml staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab75278 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
References for Anti-DOCK1 antibody (ab75278)
has not yet been referenced specifically in any publications.
Publishing research using ab75278? Please let us know so that we can cite the reference in this datasheet.
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