The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 215 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
Belongs to the DOCK family. Contains 1 DHR-1 (CZH-1) domain. Contains 1 DHR-2 (CZH-2) domain. Contains 1 SH3 domain.
The DHR-2 domain is necessary and sufficient for the GEF activity.
Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
All lanes : Anti-DOCK1 antibody (ab76927) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 215 kDa Observed band size: 215 kDa
Exposure time: 3 minutes
Immunoprecipitation - DOCK1 antibody (ab76927)
Detection of DOCK1 by Western Blot of Immunprecipitate.
ab76927, at 1µg/ml, staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab76927 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.